OTHER POSITIONS
Adjunct Faculty, Roane State Community College, Harriman, Tennessee (since 1998)
PREVIOUS EMPLOYMENT
- Senior Research Associate, Center for Environmental Biotechnology, University of Tennessee, Knoxville (1998-2001)
- Alexander Hollaender Postdoctoral Fellow, Oak Ridge National Laboratory (1997-1998)
- Graduate Research Assistant, Oklahoma State University, Stillwater, Oklahoma (1993-1996)
- Laboratory Manager, Loyola University Medical Center, Chicago, Illinois (1989-1993)
RESEARCH ACTIVITY
- Co-Principle Investigator, NASA, “Prototype biophotonic biosensor for monitoring VOC contaminants in spacecraft habitats” (2004-2006)
- Co-Principle Investigator, NASA, “Bioluminescent monitoring of opportunistic pathogens in the spacecraft environment” (2003-2005)
- Co-Principle Investigator, US-Egypt Joint Board on Scientific and Technology Cooperation, “Construction of a genetically modified biosensor to monitor nitrite toxicity in biological wastewater treatment plants” (2003-2005)
- Co-Principle Investigator, Dynamac Corporation, “Bioluminescent monitoring of bacterial pathogens” (2001)
- Co-Principle Investigator, USDA, “A novel whole-cell bioreporter model for foodborne toxin monitoring” (2001-2003)
- Co-Principle Investigator, NASA, “Towards intelligent distributed bioluminescence detection of biological contamination and stressors” (1999-2001)
HONORS AND AWARDS
- Alexander Hollaender Fellowship, Oak Ridge National Laboratory, 1997-1998
- Edward A. Grula Fellowship, Oklahoma State University, 1995-1996
SERVICE
- Review panels: NASA Yeast Model Organisms (July 2004), DOE Natural and Accelerated Bioremediation Research (May 2004)
- Ad hoc reviewer: Applied and Environmental Microbiology, Journal of Water Research, Environmental Science & Technology, Microbial Ecology, Benjamin Cummings Press, Maryland Sea Grant Institute
- Instructor: Advanced Microbial Genetics, Introduction to Microbiology
- Graduate student research committee and defense committee member
PATENTS
- Sayler, G.S., Ripp. S.A., Applegate, B.M. Bioluminescent biosensor device, U.S. Patent #6,544,729.
- Sayler, G.S., Simpson, M.L., Applegate, B.A., Ripp, S.A. In vivo biosensor apparatus and method of use, U.S. patent #6,673,596.
- Gupta, R.K., Patterson, S.S., Sayler, G.S., Ripp, S.A. Lux expression in eukaryotic cells, U.S. Patent pending 2004-0002148.
- Sayler, G.S., Ripp, S.A., Sanseverino, J. Bioluminescent methods for direct visual detection of environmental compounds, U.S. patent pending 2003-0108980.
- Simpson, M.L., Paulus, M.J., Sayler, G.S., Applegate, B.M., Ripp, S.A. Bioluminescent bioreporter integrated circuit detection methods. U.S. patent pending 2002-023168.
- Sayler, G.S., Ripp, S.A., Applegate, B.A., Bioluminescent detection of bacterial pathogens using bacteriophage containing luxI genes and bioreporter cells containing the lux gene cassette, U.S. patent pending 2001-910360.
PUBLICATIONS
1. Kuncova, G., O. Podrazky, S. Ripp, J. Trogl, G. Sayler, K. Demnerova, R. Vankova. Monitoring the viability of cells immobilized by the sol-gel process. Journal of Sol Gel Science and Technology, in press.
2. Trogl, J., S. Ripp, G. Kuncova, G. Sayler, A. Churava, P. Parik, K. Demnerova, J. Halova, L. Kubicova. Selectivity of whole cell optical biosensor with immobilized bioreporter Pseudomonas fluorescens HK44. Sensors and Actuators B, in press.
3. Ripp, S., J. Garland, G. Sayler. 2004. Bioluminescent bioreporter integrated circuit sensing of the chemical and biological spacecraft environment. Luminescence 19:170-171.
4. Gupta, R.K., S.S. Patterson, S. Ripp, G.S. Sayler. 2003. Expression of the Photorhabdus luminescens lux genes (luxA, B, C, D, and E) in Saccharomyces cerevisiae. FEMS Yeast Research 4:305-313.
5. Ripp, S., K.A. Daumer, T. McKnight, L.H. Levine, J.L. Garland, M.L. Simpson, G.S. Sayler. 2003. Bioluminescent bioreporter integrated circuit sensing of microbial volatile organic compounds. Journal of Industrial Microbiology and Biotechnology, 30:636-642.
6. Trogl, J., S. Ripp, G. Kuncova, G.S. Sayler, K. Demnerova. 2003. Bioreporter Pseudomonas fluorescens HK44 immobilized in a silica matrix. Hemijska Industrija 57:596-599.
7. Abd-El-Haleem, D., S. Ripp, C. Scott, G.S. Sayler. 2002. A luxCDABE-based bioluminescent bioreporter for the detection of phenol. Journal of Industrial Microbiology and Biotechnology 29:233-237.
8. Bolton, E.K., G.S. Sayler, D.E. Nivens, J.M. Rochelle, S. Ripp, M.L. Simpson. 2002. Integrated CMOS photodetectors and signal processing for very low-level chemical sensing with the bioluminescent bioreporter integrated circuit. Sensors and Actuators B 85:179-185.
9. Ripp, S., D.E. Nivens, C. Werner, G.S. Sayler. 2001. Vertical transport of a field-released genetically engineered microorganism through soil. Soil Biology & Biochemistry 33:1873-1877.
10. Sayler, G.S., S. Ripp, D. Nivens, M. Simpson. 2001. Bioluminescent bioreporter integrated circuits: sensing analytes and organisms with living microorganisms. Journal of Environmental Biotechnology 1:33-39.
11. Simpson, M.L., G.S. Sayler, G. Patterson, D.E. Nivens, E. Bolton, J. Rochelle, C. Arnott, B. Applegate, S. Ripp, M.A. Guillorn. 2001. An integrated CMOS microluminometer for low-level luminescence sensing in the bioluminescent bioreporter integrated circuit. Sensors and Actuators B 72:135-141.
12. Cox, C., D.E. Nivens, S. Ripp, M.M. Wong, A. Palumbo, R.S. Burlage, G.S. Sayler. 2000. An intermediate-scale lysimeter facility for subsurface bioremediation research. Bioremediation Journal 4:69-79.
13. Ripp, S., D.E. Nivens, Y. Ahn, C. Werner, J. Jarrell, J. Easter, R. Burlage, G.S. Sayler. 2000. Controlled field release of a bioluminescent genetically engineered microorganism for bioremediation process monitoring and control. Environmental Science and Technology 34:846-853.
14. Ripp, S., C. Werner, D.E. Nivens, G.S. Sayler. 2000. Most-probable-number enumeration of lux-based bioluminescent microorganisms. Applied Microbiology and Biotechnology 53:736-741.
15. Sayler, G.S., S. Ripp. 2000. Field applications of genetically modified bacteria for bioremediation processes. Current Opinion in Biotechnology 11:286-289.
16. Ripp, S., R.V. Miller. 1998. Dynamics of the pseudolysogenic response in slowly growing cells of Pseudomonas aeruginosa. Microbiology 144:2225-2232.
17. Simpson, M.L., G.S. Sayler, S. Ripp, D.E. Nivens, B.M. Applegate, M.J. Paulus, G.E. Jellison Jr. 1998. Bioluminescent bioreporter integrated circuits form novel whole-cell biosensors. Trends in Biotechnology 16:332-338.
18. Stapleton, R.D., S. Ripp, L. Jimenez, S. Cheol-Koh, J.T. Fleming, I.R. Gregory, G.S. Sayler. 1998. Nucleic acid analytical approaches in bioremediation: site assessment and characterization. Journal of Microbiological Methods 32:165-178.
19. Ripp, S., R.V. Miller. 1997. The role of pseudolysogeny in bacteriophage-host interactions in a natural freshwater environment. Microbiology 143:2065-2070.
20. Ripp, S., R.V. Miller. 1995. Effects of suspended particulates on the frequency of transduction among Pseudomonas aeruginosa in a freshwater environment. Applied and Environmental Microbiology 61:1214-1219.
21. Kidambi, S.P., S. Ripp, R.V. Miller. 1994. Evidence for phage-mediated gene transfer among Pseudomonas aeruginosa strains on the phylloplane. Applied and Environmental Microbiology 60:496-500.
22. Ripp, S., O.A. Ogunseitan, R.V. Miller. 1994. Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1. Molecular Ecology 3:121-126.
23. Quinn, J.P., A. Darzins, D. Miyashiro, S. Ripp, R.V. Miller. 1991. Imipenem resistance in Pseudomonas aeruginosa PAO: mapping of the OprD2 gene. Antimicrobial Agents and Chemotherapy 35:753-755.
BOOK CHAPTERS
1. Ripp, S. Bioreporter technology for monitoring soil bioremediation. In R. Margesin and F. Schinner (eds.), Soil Biology, Springer-Verlag, New York, NY, in press.
2. O’Neill, S., S. Ripp, C. Megginson, I.M. Davies. 2003. Microbial detection of polycyclic aromatic hydrocarbons in contaminated marine sediment. Fisheries Research Services, Aberdeen, UK.
3. Ripp, S., G.S. Sayler. 2002. Field release of bacteria. In Bitton, G. (ed.), The Encyclopedia of Environmental Microbiology, Wiley Publishers, New York, NY.
4. Ripp, S., G.S. Sayler. 2002. Microbial biodegradation and bioelectronic sensing of polyaromatic hydrocarbon in the environment, p. 237-244. In Y. A. Al-Shayji, J. S. Sidhu, M. Saleem, and K. Guerinik (ed.), Biotechnology Applications for the Arid Regions, Kuwait Institute for Scientific Research, Kuwait.
5. Miller, R.V., S.A. Ripp. 2001. Pseudolysogeny: A bacteriophage strategy for increasing longevity in situ, p. 79-89. In M. Syvanen and C.I. Kado (ed.), Horizontal Gene Transfer, Academic Press, London.
6. Hay, A., S. Ripp, G.S. Sayler. 2000. Catabolic plasmids, p. 730-744. In L. Lederberg (ed.), The Encyclopedia of Microbiology, 2nd edition, Academic Press, San Diego, CA.
7. Ripp, S., B.M. Applegate, D.E. Nivens, M.J. Paulus, G.E. Jellison, M.L. Simpson, G.S. Sayler. 2000. Whole-cell environmental monitoring devices: bioluminescent bioreporter integrated circuits (BBICs), p. 197-205. In A. Mulchandani and O.A. Sadik (ed.), Recent Advances in Environmental Chemical Sensors and Biosensors, ACS Press, Clarendon Hills, IL.
8. Ripp, S., D.E. Nivens, R. Burlage, G.S. Sayler. 1999. Field-scale bioremediation monitoring utilizing bioluminescent genetically engineered microorganisms, p. 277-282. In A. Leeson and B.C. Alleman (ed.), Bioremediation Technology for Polycyclic Aromatic Hydrocarbon Compounds, Battelle Press, Columbus, OH.
9. Miller, R.V., S. Ripp. 1998. The importance of pseudolysogeny to transduction in situ. In M. Syvane and C. Kado (ed.), Horizontal Gene Transfer, Chapman and Hall, London.
10. Ripp, S., R.V. Miller. 1993. Influence of suspended particulates on increased transduction rates among Pseudomonas aeruginosa in a freshwater ecosystem. In ORD Biotechnology Risk Assessment Program (eds.), Risk Assessment for Environmental Releases of Biotechnology Products, USEPA, Washington, DC.
11. Miller, R.V., S. Ripp, J. Replicon, O. Ogunseitan, T.A. Kokjohn. 1992. Virus-mediated gene transfer in freshwater environments, p. 50-62. In M.J. Gauthier (ed.), Gene Transfers and Environment, Springer-Verlag, Berlin, Germany.
PROCEEDINGS AND PRESENTATIONS
Over 40 presentations at various national and international venues.